沙(sha)門(men)(men)(men)氏(shi)顯色(se)(se)培養(yang)(yang)基的優點在于(yu)其快速(su)、準確、靈(ling)(ling)敏(min)度(du)高，可(ke)以很(hen)大程度(du)縮短檢(jian)(jian)測時(shi)(shi)間(jian)，提(ti)(ti)高檢(jian)(jian)測效(xiao)率。同時(shi)(shi)，該培養(yang)(yang)基對(dui)沙(sha)門(men)(men)(men)氏(shi)菌(jun)(jun)具(ju)有很(hen)高的特(te)異性(xing)(xing)，能夠有效(xiao)地避免假陽性(xing)(xing)結果(guo)的出現，使得(de)(de)檢(jian)(jian)測結果(guo)更加(jia)可(ke)靠。此外，沙(sha)門(men)(men)(men)氏(shi)顯色(se)(se)培養(yang)(yang)基還可(ke)以進行改(gai)良或(huo)增(zeng)加(jia)營養(yang)(yang)成(cheng)份(fen)以獲得(de)(de)較佳的檢(jian)(jian)測效(xiao)果(guo)。例(li)如，可(ke)以添加(jia)一些特(te)殊營養(yang)(yang)物質，如血清(qing)、酵母提(ti)(ti)取物等，以提(ti)(ti)高沙(sha)門(men)(men)(men)氏(shi)菌(jun)(jun)的生長(chang)速(su)度(du)和(he)菌(jun)(jun)落數(shu)量；也可(ke)以添加(jia)一些抗笙素等物質，以抑制其他雜菌(jun)(jun)的生長(chang)，提(ti)(ti)高檢(jian)(jian)測的特(te)異性(xing)(xing)。沙(sha)門(men)(men)(men)氏(shi)顯色(se)(se)培養(yang)(yang)基是(shi)一種(zhong)非常實用(yong)的食源性(xing)(xing)致(zhi)病菌(jun)(jun)檢(jian)(jian)測工(gong)具(ju)，具(ju)有普遍的應用(yong)前景和(he)市場前景。未(wei)來可(ke)以通過進一步的研發和(he)技術改(gai)進，不斷提(ti)(ti)高其靈(ling)(ling)敏(min)度(du)和(he)特(te)異性(xing)(xing)，從(cong)而更好地保障人(ren)類食品安全(quan)和(he)健(jian)康。沙(sha)門(men)(men)(men)氏(shi)菌(jun)(jun)顯色(se)(se)培養(yang)(yang)基是(shi)一種(zhong)專門(men)(men)(men)用(yong)于(yu)檢(jian)(jian)測沙(sha)門(men)(men)(men)氏(shi)菌(jun)(jun)的培養(yang)(yang)基。廣州沙(sha)門(men)(men)(men)氏(shi)菌(jun)(jun)顯色(se)(se)培養(yang)(yang)基簡介

沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)+顯色(se)培(pei)養(yang)(yang)(yang)基(ji)：用(yong)(yong)于沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)（傷寒(han)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)和(he)(he)(he)乳糖(tang)(tang)陽(yang)性(xing)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)）的(de)(de)(de)(de)(de)分離、檢測(ce)一(yi)般認為可致(zhi)(zhi)病的(de)(de)(de)(de)(de)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)是(shi)乳糖(tang)(tang)陰性(xing)菌(jun)，但隨(sui)著可致(zhi)(zhi)病的(de)(de)(de)(de)(de)乳糖(tang)(tang)陽(yang)性(xing)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)的(de)(de)(de)(de)(de)增(zeng)加，ISO6579:2002 要求(qiu)(qiu)不斷(duan)提高中毒食(shi)品樣品中乳糖(tang)(tang)陽(yang)性(xing)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)的(de)(de)(de)(de)(de)檢出(chu)率。傳統(tong)的(de)(de)(de)(de)(de)檢測(ce)方(fang)法(fa)會出(chu)現乳糖(tang)(tang)陽(yang)性(xing)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)的(de)(de)(de)(de)(de)漏檢現象(xiang)(xiang)，將不能(neng)滿(man)足(zu)ISO6579:2002的(de)(de)(de)(de)(de)要求(qiu)(qiu)，科(ke)瑪(ma)嘉沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)快檢方(fang)法(fa)恰(qia)(qia)好(hao)彌(mi)補了這(zhe)一(yi)缺(que)陷，能(neng)簡單的(de)(de)(de)(de)(de)鑒別沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)屬包(bao)括乳糖(tang)(tang)陽(yang)性(xing)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)、傷寒(han)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)和(he)(he)(he)副傷寒(han)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)傳統(tong)的(de)(de)(de)(de)(de)檢測(ce)方(fang)法(fa)會出(chu)現乳糖(tang)(tang)陽(yang)性(xing)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)的(de)(de)(de)(de)(de)漏檢現象(xiang)(xiang)，將不能(neng)滿(man)足(zu)ISO6579:2002的(de)(de)(de)(de)(de)要求(qiu)(qiu)，科(ke)瑪(ma)嘉沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)快檢方(fang)法(fa)恰(qia)(qia)好(hao)彌(mi)補了這(zhe)一(yi)缺(que)陷，能(neng)簡單的(de)(de)(de)(de)(de)鑒別沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)屬包(bao)括乳糖(tang)(tang)陽(yang)性(xing)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)、傷寒(han)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)和(he)(he)(he)副傷寒(han)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)。西安(an)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)+顯色(se)培(pei)養(yang)(yang)(yang)基(ji)簡介沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)顯色(se)培(pei)養(yang)(yang)(yang)基(ji)是(shi)一(yi)種用(yong)(yong)于快速檢測(ce)沙(sha)(sha)(sha)(sha)(sha)(sha)門(men)(men)氏(shi)(shi)(shi)(shi)(shi)菌(jun)的(de)(de)(de)(de)(de)顯色(se)培(pei)養(yang)(yang)(yang)基(ji)。

沙門氏菌(jun)顯(xian)色培(pei)養基(ji)(ji)使用說明書：操作：1、取瓶(ping)內干粉34.9g溶(rong)于1000 mL去離(li)子水的潔凈三角(jiao)瓶(ping)中，充分(fen)攪(jiao)(jiao)拌混勻。也可根(gen)據需要按(an)照34.9 g/L的比例擴大或縮小(xiao)制備(bei)培(pei)養基(ji)(ji)的量(liang)。2、加(jia)(jia)(jia)熱(re)(re)(re)至100℃，不停攪(jiao)(jiao)拌，使其完全溶(rong)解。切(qie)勿(wu)(wu)(wu)加(jia)(jia)(jia)熱(re)(re)(re)超過100℃，切(qie)勿(wu)(wu)(wu)121℃高壓滅菌(jun)。若使用微波爐加(jia)(jia)(jia)熱(re)(re)(re)，應將培(pei)養基(ji)(ji)加(jia)(jia)(jia)熱(re)(re)(re)沸騰，立即移出(chu)，輕輕搖勻，再放入微波爐加(jia)(jia)(jia)熱(re)(re)(re)，觀察小(xiao)氣泡變為大氣泡，直至完全溶(rong)解即可。切(qie)勿(wu)(wu)(wu)使培(pei)養基(ji)(ji)溢(yi)出(chu)。3、加(jia)(jia)(jia)熱(re)(re)(re)后的培(pei)養基(ji)(ji)冷(leng)卻至45℃50℃，輕輕地搖動均(jun)勻，傾注平皿(min)，使其凝(ning)固，晾(liang)干備(bei)用。

沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)顯(xian)色(se)(se)培(pei)(pei)養(yang)(yang)(yang)基(ji)(ji)可(ke)(ke)以(yi)檢測(ce)出沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)菌(jun)(jun)(jun)(jun)的(de)(de)(de)(de)(de)抗笙素(su)敏(min)感性。沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)菌(jun)(jun)(jun)(jun)染(ran)上可(ke)(ke)以(yi)使用多種抗笙素(su)進行(xing)醫(yi)治，但不同的(de)(de)(de)(de)(de)菌(jun)(jun)(jun)(jun)株對抗笙素(su)的(de)(de)(de)(de)(de)敏(min)感性不同。沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)顯(xian)色(se)(se)培(pei)(pei)養(yang)(yang)(yang)基(ji)(ji)中(zhong)可(ke)(ke)以(yi)添加不同的(de)(de)(de)(de)(de)抗笙素(su)，可(ke)(ke)以(yi)檢測(ce)出沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)菌(jun)(jun)(jun)(jun)對不同抗笙素(su)的(de)(de)(de)(de)(de)敏(min)感性，從而幫助醫(yi)生選(xuan)擇較有效的(de)(de)(de)(de)(de)醫(yi)治方案(an)。沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)顯(xian)色(se)(se)培(pei)(pei)養(yang)(yang)(yang)基(ji)(ji)可(ke)(ke)以(yi)用于食(shi)品(pin)(pin)和(he)(he)(he)水(shui)樣的(de)(de)(de)(de)(de)檢測(ce)。沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)菌(jun)(jun)(jun)(jun)是一(yi)(yi)種常見的(de)(de)(de)(de)(de)食(shi)源(yuan)性的(de)(de)(de)(de)(de)病(bing)原菌(jun)(jun)(jun)(jun)，可(ke)(ke)以(yi)通(tong)過食(shi)品(pin)(pin)和(he)(he)(he)水(shui)傳播。沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)顯(xian)色(se)(se)培(pei)(pei)養(yang)(yang)(yang)基(ji)(ji)可(ke)(ke)以(yi)用于檢測(ce)食(shi)品(pin)(pin)和(he)(he)(he)水(shui)中(zhong)的(de)(de)(de)(de)(de)沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)菌(jun)(jun)(jun)(jun)，從而保(bao)障公眾的(de)(de)(de)(de)(de)健(jian)康和(he)(he)(he)安全。沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)顯(xian)色(se)(se)培(pei)(pei)養(yang)(yang)(yang)基(ji)(ji)是一(yi)(yi)種常用的(de)(de)(de)(de)(de)微生物培(pei)(pei)養(yang)(yang)(yang)基(ji)(ji)，具有區分沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)菌(jun)(jun)(jun)(jun)和(he)(he)(he)其他革蘭氏(shi)(shi)陰性菌(jun)(jun)(jun)(jun)、檢測(ce)沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)菌(jun)(jun)(jun)(jun)的(de)(de)(de)(de)(de)不同血(xue)清型(xing)和(he)(he)(he)抗笙素(su)敏(min)感性、以(yi)及用于食(shi)品(pin)(pin)和(he)(he)(he)水(shui)樣的(de)(de)(de)(de)(de)檢測(ce)等優點。在臨(lin)床和(he)(he)(he)公共衛生領域，沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)顯(xian)色(se)(se)培(pei)(pei)養(yang)(yang)(yang)基(ji)(ji)具有重要的(de)(de)(de)(de)(de)應用價值。該培(pei)(pei)養(yang)(yang)(yang)基(ji)(ji)含有特(te)殊(shu)營養(yang)(yang)(yang)物質和(he)(he)(he)混合(he)顯(xian)色(se)(se)劑，能夠為沙(sha)(sha)(sha)(sha)(sha)門(men)氏(shi)(shi)菌(jun)(jun)(jun)(jun)提供較佳的(de)(de)(de)(de)(de)生長環境。

沙門(men)(men)氏顯(xian)(xian)色培養(yang)(yang)(yang)(yang)基在實(shi)(shi)驗(yan)(yan)室中(zhong)(zhong)樣品污(wu)(wu)(wu)染(ran)問(wen)題(ti)(ti)：在樣品采集(ji)(ji)、處(chu)理和接種過(guo)(guo)程(cheng)中(zhong)(zhong)，可(ke)能(neng)會因(yin)(yin)為操作不(bu)(bu)規范(fan)、設備污(wu)(wu)(wu)染(ran)、環(huan)境(jing)因(yin)(yin)素(su)等(deng)導(dao)致樣品受到污(wu)(wu)(wu)染(ran)。例(li)如，采集(ji)(ji)的(de)(de)(de)樣品被其他雜菌(jun)污(wu)(wu)(wu)染(ran)，或接種設備的(de)(de)(de)清(qing)潔度不(bu)(bu)足。應(ying)對(dui)策略：嚴格遵守樣品采集(ji)(ji)和處(chu)理的(de)(de)(de)操作規程(cheng)，確保采集(ji)(ji)的(de)(de)(de)樣品符合(he)要求。同(tong)時(shi)，對(dui)實(shi)(shi)驗(yan)(yan)環(huan)境(jing)和設備進(jin)行(xing)有效的(de)(de)(de)清(qing)潔和消毒，避免交叉污(wu)(wu)(wu)染(ran)。沙門(men)(men)氏顯(xian)(xian)色培養(yang)(yang)(yang)(yang)基在實(shi)(shi)驗(yan)(yan)室中(zhong)(zhong)培養(yang)(yang)(yang)(yang)條件(jian)問(wen)題(ti)(ti)：在培養(yang)(yang)(yang)(yang)過(guo)(guo)程(cheng)中(zhong)(zhong)，可(ke)能(neng)會因(yin)(yin)為溫度、濕度、時(shi)間等(deng)參數控制不(bu)(bu)當而導(dao)致檢(jian)測結果不(bu)(bu)準(zhun)確。例(li)如，培養(yang)(yang)(yang)(yang)溫度過(guo)(guo)高或過(guo)(guo)低，濕度不(bu)(bu)足或過(guo)(guo)度等(deng)。應(ying)對(dui)策略：根據(ju)實(shi)(shi)驗(yan)(yan)要求設定合(he)適(shi)的(de)(de)(de)培養(yang)(yang)(yang)(yang)條件(jian)，并進(jin)行(xing)實(shi)(shi)時(shi)監控。同(tong)時(shi)，定期對(dui)培養(yang)(yang)(yang)(yang)設備進(jin)行(xing)檢(jian)查和維護，確保培養(yang)(yang)(yang)(yang)條件(jian)的(de)(de)(de)穩定性。沙門(men)(men)氏菌(jun)顯(xian)(xian)色培養(yang)(yang)(yang)(yang)基的(de)(de)(de)檢(jian)驗(yan)(yan)原(yuan)理是利用混合(he)色素(su)與沙門(men)(men)氏菌(jun)和大腸(chang)菌(jun)群所對(dui)應(ying)的(de)(de)(de)酶發(fa)生特異性反應(ying)。重慶沙門(men)(men)氏+顯(xian)(xian)色培養(yang)(yang)(yang)(yang)基研究

使(shi)用過的(de)沙門(men)氏菌顯色培養(yang)基(ji)需(xu)要在一定(ding)溫度下進行(xing)滅菌處理，然后才可按(an)照規定(ding)丟棄。廣州沙門(men)氏菌顯色培養(yang)基(ji)簡介

沙門(men)氏菌檢(jian)(jian)測過程進(jin)行(xing)分析(xi)梳理(li)(li)之血(xue)(xue)清學鑒定：（1）以無菌干凈的(de)玻(bo)片(pian)(pian)為載(zai)體，滴(di)一(yi)(yi)滴(di)生理(li)(li)鹽水，接(jie)種管(guan)從營養瓊脂平(ping)面挑取少(shao)量菌體，在(zai)(zai)生理(li)(li)鹽水中(zhong)涂開，緩緩涂，觀察凝(ning)集(ji)情況(kuang)，排除(chu)自凝(ning)。（2）滴(di)一(yi)(yi)滴(di)O多價血(xue)(xue)清于(yu)(yu)玻(bo)片(pian)(pian)上(shang)，重復上(shang)述步驟(zou)，判斷凝(ning)集(ji)情況(kuang)，一(yi)(yi)般情況(kuang)都會(hui)凝(ning)集(ji)的(de)。除(chu)非是(shi)有Vi抗原存在(zai)(zai)時（如鼠傷寒沙門(men)氏菌），會(hui)阻止O血(xue)(xue)清凝(ning)集(ji)，可挑取菌體至1 mL生理(li)(li)鹽水中(zhong)制成菌懸液，于(yu)(yu)酒精(jing)燈煮(zhu)沸(fei)在(zai)(zai)檢(jian)(jian)查。（3）滴(di)一(yi)(yi)滴(di)H多價血(xue)(xue)清于(yu)(yu)玻(bo)片(pian)(pian)上(shang)，重復上(shang)述（1）中(zhong)步驟(zou)，判斷凝(ning)集(ji)情況(kuang)。若凝(ning)集(ji)效果不明顯，需(xu)要用0.6%半固(gu)體瓊脂平(ping)板誘導，帶菌落蔓(man)延(yan)生長是(shi)，挑取邊(bian)緣部分進(jin)行(xing)檢(jian)(jian)查。廣(guang)州沙門(men)氏菌顯色培養基簡介(jie)